Gelacell™
Pharma-grade 3D nanofibrous scaffolds for advanced 3D cell culture and tissue engineering
Taking cell culture to the next dimension
Gelacell™
3D nanofibrous scaffold
Gelacell™ is a non-woven highly porous scaffold specially designed for in vitro 3D cell culture and tissue engineering. It has a unique nanofibrous structure that closely mimics the natural extracellular matrix.
Designed as a non-woven, highly porous scaffold, Gelacell™
offers exceptional biocompatibility and non-toxicity across a variety of cell types.
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Gelacell™ is produced using patented halospinning technology that provides unprecedented 3D architecture with extensive surface area for cellular activities. Compatible with various cell lines and culture conditions.
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Morphological benefits of halospun nanofibers
High surface-to-volume ratio
Halospun nanofibers have an extremely high surface-to-volume ratio, which enhances cell attachment, growth, and differentiation.
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Porosity
The interconnected porous structure of these nanofibers facilitates efficient nutrient and oxygen transport to cells, which is critical for cell survival and proliferation.
Biomimicry
Nanofibers, due to their scale and structure, can effectively mimic the extracellular matrix (ECM) of various tissues, providing a more natural environment for cells.
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Customizability
Fiber diameter, alignment, and density can be controlled during the halospinning process, allowing for customization to suit specific cell types and applications.
Enhanced cell interactions
Due to their morphology, halospun nanofibers promote superior cell-to-cell and cell-to-fiber interactions, encouraging the formation of 3D cell networks.
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Flexibility in material selection
Halospun nanofibers can be made from a wide variety of polymers, both natural and synthetic, allowing for a range of mechanical and chemical properties to suit different cell culture needs.
The 3D structure created by halospinning closely mimics the natural extracellular matrix
Extracellular matrix
Source: Mdpi.com, Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver, H.Ijima, S. Nakamura, R. Bual, etc. 2018
Gelatex halospun nanofibers
Electrospun nanofibers
Source: Gelatex
Source: Gelatex
Gelacell™ 3D advantages
vs conventional 2D systems
Acts as an in-vitro extracellular matrix
Preserves the natural cell structure
Enhances cell-to-cell and cell-to-matrix interactions
Supports optimal
cell differentiation
Porous structure facilitates nutrient diffusion
Protects cells during laboratory practices
Maintain the in vivo morphology of your cells and improve the relevance of your research
In traditional 2D in vitro systems, cells tend to flatten and stretch in a monolayer, creating stress and modifying their natural behavior
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A 3D in vitro scaffold helps preserve the natural shape of the cells, reduces the stress conditions, and allows for the structural conformation found in native tissues. In a 3D system, cells retain more of their original functions, surface activity, and natural complex interactions
Morphological differences
2D vs 3D
cell culture
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Gelacell™ 3D nanofibrous scaffold stands at an intersection
of hard scaffold and hydrogel properties.
The nanofibers provide a perfect balance of rigidity and flexibility, allowing for high cell interaction while being easy to handle and store, making it an unparalleled choice in the field of 3D cell culture scaffolds.
3D structures that facilitate enhanced cell migration and proliferation.
Exceptional morphological characteristics that mimic the natural ECM.
Versatile applications in 3D cell culture and tissue engineering.
High batch-to-batch reproducibility, user-friendly handling, and storage at room temperature.
Gelacell™ is a perfect solution for transitioning from hydrogel and electrospun scaffolds.
Possible applications for Gelacell™
Cardiotoxicology
Aligned scaffolds improve both structural and functional read-outs in cardiomyocytes, growing 3D cultures of spontaneously beating hiPSC-derived cardiomyocytes (hiPSC-CMs) in well format. hiPSC-CMs grown on such aligned 3D plates showed statistically significantly higher Ca2+ transient rising slope (indicating faster kinetics), lower peak width durations, and lower amplitudes as compared to standard 2D tissue culture plates
Toxicology studies
Hep G2 liver cancer cells are often used as model cultures for toxicology studies in-vitro. Nanofiber scaffolds have proven to provide a suitable environment for liver cells.
Drug discovery in 3D tissue model
Cancer cells grown in more physiologically relevant 3D cultures have shown increased drug resistance compared to traditional 2D systems. Nanofiber scaffolds have been successfully used as a matrix for numerous cancer cell models in 3D drug screening: liver, breast, ovarian as well as lung cancer models.
Stem cell research
Differentiation of neural stem cells into mature neurons within nanofiber scaffolds.
3D cell culture studies
with Gelacell™
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Wide range of cell seeding density/well (10^4 to 10^6).
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Progressive growth of cells in the 3D environment.
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Activity assay’s compatible but not limited to:
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MTT
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MTS
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CCK8
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Dyes compatible with the scaffold:
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Calcein AM/Propidium Iodide (Live/dead)
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Phalloidin conjugates
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FDA
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DAPI/Hoechst
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Direct analysis of activity assays under well plate reader.
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Scaffold preserving natural cellular structure.
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Growth of fibroblast cells (BHK21) on Gelacell™ halospun PLGA scaffolds in comparison with cells on the bottom of the well plate (2D well) using CCK8 assay
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Growth of myoblast cells (C2C12) on Gelacell™ halospun PLGA scaffolds in comparison with cells on the bottom of the well plate (2D well) using CCK8 assay
The synergistic contribution of stiffness and porosity lead to
cell migration, proliferation, and differentiation.
Read the white paper to learn more about Gelacell™
Gelacell™ 3D nanofibrous scaffolds
are available for purchase
Well plate with scaffold
24 well plate with scaffolds fixed on PET discs.
Meant for short to medium term 3D cell culture. PET discs enable easy handling.
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Available as 24 PLLA well plate with randomly oriented fibers or aligned.
For more detailed information and data sheets of currently available products please see our product catalogue.
Well plate with cell crowns
6, 12 and 24 well plates with scaffolds on cell crowns
Meant for long term 3D cell culture.
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Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.
For more detailed information and data sheets of currently available products please see our product catalogue.
Inserts
Scaffold inserts to fit into existing 6, 12 and 24 well plates
Meant for short to medium term 3D cell culture.
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Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.
For more detailed information and data sheets of currently available products please see our product catalogue.
Scaffold sheet
10 x 10 cm single scaffold sheets
Meant for tissue engineering.
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Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.
For more detailed information and data sheets of currently available products please see our product catalogue.
Committed to quality and safety
All products are manufactured in state-of-the-art ISO Class 7 level cleanrooms, ensuring that the risk of microbial and particulate contamination is minimized. This provides a controlled environment that significantly reduces the number of airborne particles, facilitating the production of high-quality, safe products.
Our upcoming ISO 13485 certification, coupled with our current cleanroom production standards, underlines our promise to deliver products that meet and exceed international quality and safety benchmarks.