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Gelacell™

Pharma-grade 3D nanofibrous scaffolds for advanced 3D cell culture and tissue engineering

Taking cell culture to the next dimension

Gelacell by Gelatex 10x10 scaffold package

Gelacell™
3D nanofibrous scaffold

Gelacell is a non-woven highly porous scaffold specially designed for in vitro 3D cell culture and tissue engineering. It has a unique nanofibrous structure that closely mimics the natural extracellular matrix.

 

Designed as a non-woven, highly porous scaffold, Gelacell™
offers exceptional biocompatibility and non-toxicity across a variety of cell types.

Gelacell is produced using patented halospinning technology that provides unprecedented 3D architecture with extensive surface area for cellular activities. Compatible with various cell lines and culture conditions.

Morphological benefits of halospun nanofibers

High surface-to-volume ratio

Halospun nanofibers have an extremely high surface-to-volume ratio, which enhances cell attachment, growth, and differentiation.

Porosity

The interconnected porous structure of these nanofibers facilitates efficient nutrient and oxygen transport to cells, which is critical for cell survival and proliferation.

Biomimicry

Nanofibers, due to their scale and structure, can effectively mimic the extracellular matrix (ECM) of various tissues, providing a more natural environment for cells.

Customizability

Fiber diameter, alignment, and density can be controlled during the halospinning process, allowing for customization to suit specific cell types and applications.

Enhanced cell interactions

Due to their morphology, halospun nanofibers promote superior cell-to-cell and cell-to-fiber interactions, encouraging the formation of 3D cell networks.

Flexibility in material selection

 Halospun nanofibers can be made from a wide variety of polymers, both natural and synthetic, allowing for a range of mechanical and chemical properties to suit different cell culture needs.

SEM image of halospun nanofibers

The 3D structure created by halospinning closely mimics the natural extracellular matrix

Extracellular matrix
Halospun nanofibers
Electrospun nanofibers

Extracellular matrix

Source: Mdpi.com, Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver, H.Ijima, S. Nakamura, R. Bual, etc. 2018

Gelatex halospun nanofibers

Electrospun nanofibers

Source: Gelatex

Source: Gelatex

Gelacell™ 3D advantages
vs conventional 2D systems

Acts as an in-vitro extracellular matrix

Preserves the natural cell structure

Enhances cell-to-cell and cell-to-matrix interactions

Supports optimal
cell differentiation

Porous structure facilitates nutrient diffusion

Protects cells during laboratory practices

Maintain the in vivo morphology of your cells and improve the  relevance of your research

In traditional 2D in vitro systems, cells tend to flatten and stretch in a monolayer, creating stress and modifying their natural behavior

A 3D in vitro scaffold helps preserve the natural shape of the cells, reduces the stress conditions, and allows for the structural conformation found in native tissues. In a 3D system, cells retain more of their original functions, surface activity, and natural complex interactions

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Morphological differences 

2D vs 3D
cell culture

2d vs 3d replacement with ref (1)_edited

Gelacell™ 3D nanofibrous scaffold stands at an intersection
of hard scaffold and hydrogel properties.


The nanofibers provide a perfect balance of rigidity and flexibility, allowing for high cell interaction while being easy to handle and store, making it an unparalleled choice in the field of 3D cell culture scaffolds. 

3D structures that facilitate enhanced cell migration and proliferation.

Exceptional morphological characteristics that mimic the natural ECM.

Versatile applications in 3D cell culture and tissue engineering.

High batch-to-batch reproducibility, user-friendly handling,  and storage at room temperature.

Gelacell™ is a perfect solution for transitioning from hydrogel and electrospun scaffolds.

Gelacell by Gelatex scaffolds in well plate
Gelatex nanofibrous material macro photo

Possible applications for Gelacell

Cardiotoxicology

Aligned scaffolds improve both structural and functional read-outs in cardiomyocytes, growing 3D cultures of spontaneously beating hiPSC-derived cardiomyocytes (hiPSC-CMs) in well format. hiPSC-CMs grown on such aligned 3D plates showed statistically significantly higher Ca2+ transient rising slope (indicating faster kinetics), lower peak width durations, and lower amplitudes as compared to standard 2D tissue culture plates

 

Toxicology studies 

Hep G2 liver cancer cells are often used as model cultures for toxicology studies in-vitro. Nanofiber scaffolds have proven to provide a suitable environment for liver cells.

 

Drug discovery in 3D tissue model

Cancer cells grown in more physiologically relevant 3D cultures have shown increased drug resistance compared to traditional 2D systems. Nanofiber scaffolds have been successfully used as a matrix for numerous cancer cell models in 3D drug screening: liver, breast, ovarian as well as lung cancer models.

 

Stem cell research

Differentiation of neural stem cells into mature neurons within nanofiber scaffolds.

3D cell culture studies
with Gelacell

  • Wide range of cell seeding density/well (10^4 to 10^6).

  • Progressive growth of cells in the 3D environment.

  • Activity assay’s compatible but not limited to:

    • MTT

    • MTS

    • CCK8

  • Dyes compatible with the scaffold:

    • Calcein AM/Propidium Iodide (Live/dead)

    • Phalloidin conjugates

    • FDA

    • DAPI/Hoechst

  • Direct analysis of activity assays under well plate reader.

  • Scaffold preserving natural cellular structure.

Growth of fibroblast cells (BHK21) on halospun PLGA scaffolds in comparison with cells on

Growth of fibroblast cells (BHK21) on Gelacell™ halospun PLGA scaffolds in comparison with cells on the bottom of the well plate (2D well) using CCK8 assay

Growth of myoblast cells(C2C12) on halospun PLGA scaffolds in comparison with cells on the

Growth of myoblast cells (C2C12) on Gelacell™ halospun PLGA scaffolds in comparison with cells on the bottom of the well plate (2D well) using CCK8 assay

The synergistic contribution of stiffness and porosity lead to
cell migration, proliferation, and differentiation.

Gelacell white paper

Read the white paper to learn more about Gelacell

Gelacell white paper front page

Gelacell 3D nanofibrous scaffolds
are available for purchase

Well plate with scaffold

24 well plate with scaffolds fixed on PET discs.

Meant for short to medium term 3D cell culture. PET discs enable easy handling.

Available as 24 PLLA well plate with randomly oriented fibers or aligned.

Gelacel well plate with scaffold on PET discs

For more detailed information and data sheets of currently available products please see our product catalogue.

Well plate with cell crowns

6, 12 and 24 well plates with scaffolds on cell crowns

Meant for long term 3D cell culture.

Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.

Gelacell scaffold on cell crowns

For more detailed information and data sheets of currently available products please see our product catalogue.

Inserts

Scaffold inserts to fit into existing 6, 12 and 24 well plates

Meant for short to medium term 3D cell culture.

Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.

Gelacell 6-well plate insert

For more detailed information and data sheets of currently available products please see our product catalogue.

Scaffold sheet

10 x 10 cm single scaffold sheets 

Meant for tissue engineering.

Available from PLLA, PLGA, PLGA:PCL, PCL, gelatin and chitosan with randomly oriented fibers or aligned.

Gelacell 10x10 scaffold

For more detailed information and data sheets of currently available products please see our product catalogue.

Gelatex halospinning machine

Committed to quality and safety

All products are manufactured in state-of-the-art ISO Class 7 level cleanrooms, ensuring that the risk of microbial and particulate contamination is minimized. This provides a controlled environment that significantly reduces the number of airborne particles, facilitating the production of high-quality, safe products. 

 

Our upcoming ISO 13485 certification, coupled with our current cleanroom production standards, underlines our promise to deliver products that meet and exceed international quality and safety benchmarks.

Do you have any questions?

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